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KMID : 0359319960360040859
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Korean Journal of Veterinary Research
1996 Volume.36 No. 4 p.859 ~ p.871
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Studies on the pathogenesis of Korean isolate of Aujeszky´s disease virus in experimentally infected piglets
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김재훈/Kim, Jae Hoon
조성환/윤용덕/권창희/조우영/박최규/현방훈/Cho, Sung Whan/Yoon, Yong Dhuk/Kweon, Chang Hee/Cho, Woo Young/Park, Choi Gui/Hyun, Bang Hoon
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Abstract
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This study was conducted to elucidate the distribution of Aujeszky´s disease viral nucleic acids and antigens in the central nervous system (CNS) of piglets. The first Korean isolate of Aujeszky´s disease virus(ADV) that isolated from naturally infected piglets in Yang San, was inoculated into 32 day old piglets with 10^(5.9) TCID_(50)/㎖ through intranasal or intramuscular route. These piglets were sacrificed at every 24hrs for 8 days. The immunohistochemistry (IHC) was conducted to detect the viral antigens in paraffin-embedded tissue sections using avidin-biotin-peroxidase complex (ABC) method. The viral nucleic acids were detected by in situ hybridization (ISH) using ADV specific DNA probe labeled with digoxigenin.
The ADV antigens were detected in reticuloendothelial cells of spleen, lymph nodes and tonsil, alveolar walls, leptomeningeal vascular walls, inflammatory foci of each organ, and nerve cells.
The viral nucleic acids were detected in the spinal trigeminal nucleus and its tracts of the pons and medulla oblongata by the ISH technique.
The pathways of AD viruses in CNS were determined by IHC and ISH. In the intranasally inoculated group, the viruses in nasal mucosa moved to medulla oblongata and pons through the trigeminal nerve. In case of intramuscullarly inoculated group, viruses moved to brain via lymphoid organs or spinal nerves from sciatic nerves.
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